1
عمومی::
هوموژنات مغز
[0043] FIG. 4 illustrates the binding affinity of several anti-pS413-Tau antibodies for phosphorylated Tau in brain homogenate from an Alzheimer's patient, in accordance with some embodiments of the disclosure.
FIG. 6A shows concentration of various humanized antibodies in brain homogenate.
[0049] FIG. 10A - FIG. 10E show comparable binding characteristics of the parent mouse antibody (FIG. 10A), the chimeric antibody (FIG. 10B), and selected humanized antibody variants (FIG. 10C-10E) to the S413-phosphorylated Tau protein in brain homogenates from Alzheimer's disease patients.
, one week), collecting the blood and preparing brain homogenate, measuring the antibody concentration in each of the obtained plasma sample and the obtained brain homogenate sample using a known method (e.g.
[00269] In addition, the anti-pSer413 tau antibodies of the invention can also be assayed for binding affinities in human AD brain homogenates, as well as assayed in neutralization studies of AD-derived au seeds in a HEC293 Tau P301L aggregation assay, and/or in AD-derived tau seeding and spreading models using human iPSC neurons in microfluidic chambers.
واژگان شبکه مترجمین ایران